Description
CHST3, also known as chondroitin 6-O-sulfotransferase, is a member of sulfotransferase 1 family. The human CHST family is comprised of 14 enzymes and all members of this family are Golgi-localized type II membrane proteins. These enzymes utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. It can also sulfate Gal residues of keratan sulfate and Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. CHST3 Widely expressed in adult tissues. Expressed in heart, placenta, skeletal muscle and pancreas. Also expressed in various immune tissues such as spleen, lymph node, thymus and appendix. Recombinant human CHST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques